The pgl3 luciferase reporter vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. A highthroughput screen for genes essential for prrsv. Vector algebra 425 now observe that if we restrict the line l to the line segment ab, then a magnitude is prescribed on the line l with one of the two directions, so that we obtain a directed line segment fig 10. The legend of the yaxis of the right diagram is indicated as lg prrsv orf7 relative expression level. The pgl3 vector region from the start of the reporter gene the nco i. This vector is useful in the study of functional promoter elements to regulate gene expression because transcription of the luciferase gene is maintained at a higher level by using the sv40 enhancer. Because there should be no barriers to seeing your data. Characterization of 3untranslated region of the mouse gdnf. These factors may be cisacting, such as promoters and enhancers, or transacting, such as various dnabinding factors. Chronic obstructive pulmonary disease copd and pulmonary tuberculosis ptb share a number of common risk factors, including innate immunityrelated genetic factors. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to. Isolation and cloning of human vegf promoter region in pgl3. The vai gene was pcr amplified from padvantage and ligated into pgl3 basic, yielding pgl3vai which was transiently transfected into hela cells and demonstrated a dose response increase in luciferase. Choose from over a million free vectors, clipart graphics, vector art images, design templates, and illustrations created by artists worldwide.
Oct 22, 2019 the pgl3 basic vector without an insert sequence was used as a negative control. E and f luciferase reporter assays using vectors containing the snps locus for either promoter e or enhancer f assay. The enhancer vector contains the sv40 enhancer region for putative promoters that may need an additional regulator region. Home resources plasmid files luciferase vectors pgl4. Ligation of vegf into pgl3 basic the digested pgl3 basic.
Double digestion of vegf and pgl3 basic vector, using mlu i and bgl ii the gel purified nested pcr products which were believed to be vegf promoters the insert and also pgl3 basic vector were digested by using restriction enzymes mlu i and bgl ii. Welcome to vector database vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Isolation and cloning of human vegf promoter region in. Apr 20, 2020 converting pdf to vector directly is a challenge.
Glial cell linederived neurotrophic factor gdnf is a potent survival factor for many cell types, and its expression is widespread both within and outside of the nervous system. Risk snpmediated promoterenhancer switching drives prostate. The abo blood group system is of great importance in blood transfusion and organ transplantation. Akiyoshi fukamizu institute of applied biochemistry, university of tsukuba, tsukuba, japan. A vector file is a file illustrator, corel draw that can be opened and changed repeatedly with ease and can be sc. Regulation of expression of mlaa34 gene through transcriptional. Promoter sequence of human hsp70 gene containing functionally active hses designated. This page is informational only this vector is not available from addgene please contact the manufacturer for further details. May 31, 2012 the abo blood group system is of great importance in blood transfusion and organ transplantation.
Transcript levels of betalactamase were measured by rtpcr after transfection of different amounts of the pgl3 basic and pgl3control vectors. Human maf1 negatively regulates rna polymerase iii. Luciferase activity was detected 48 h after transfection. Identification of a novel distal enhancer in human adiponectin gene. Pdf normalization of transfection efficiency using the. Regions of interest within the gcnt1 gene locus were amplified by pcr using the primers given in a supplementary file supplementary table i and cloned into the pgl3 basic vector from promega. To determine whether this sequence confers promoter activity, several cell lines including. Functional variations of the tlr4 gene in association with. Iman yaze, razauden zulkifli, asma chinigarzadeh, reyhaneh rahnamai tajadod isolation and cloning of human vegf promoter region in pgl3 basic. The pgl3 vector region from the start of the reporter gene the nco i restriction site to the bacterial origin of replication sequence was also redesigned. Isolation and cloning of human nqo1 promoter in pgl3 basic. Here are a few examples of the vector magic output for various types of input. The regulation of gdnf expression has been extensively investigated but is not fully understood. Using a luciferase reporter assay, we identified the role of the 3untranslated region 3utr of the mouse gdnf gene.
This vector is typically used to study upstram or downstream enhancer elements, but not promoters as there is already a promoter, tata box and other elements to run basic. Hipdf is a tool that will help users convert pdf to various file. The human gene for liver and red blood cellspecific pk pklr is located on chromosome 1q21 3 where it directs tissuespecific transcription for both the liverspecific isozymes pkl and the red blood. The originals have been uploaded to and processed by this website, using only the tools made available in the vectorization wizard. Comparison of the responsiveness of the pgl3 and pgl4 luciferase. Expression of luciferase activity in cells transfected with this. Hipdf is a tool that will help users convert pdf to various file formats, vice versa. The reporter vectors are available in four options, depending on your needs. The basic vector has the reporter gene and restriction enzymes sites to clone in a promoter of interest. Three pgl4 reporter vectors that express luciferase proteins with different stabilities for investigating gene expression control regions. This is a free resource for the scientific community that is compiled by addgene this page is informational only this vector is not available from addgene please contact the manufacturer for further details. A new packaging cell line for sv40 vectors that eliminates. Promoter region sequence of generated vectors was confirmed by restriction digestion. The pgl3 basic vector lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.
Ligation of vegf into pgl3 basic the digested pgl3 basic vector was treated with calf. Iman yaze, razauden zulkifli, asma chinigarzadeh, reyhaneh rahnamai tajadod isolation and cloning of human vegf promoter region in pgl3 basic vector published at. These results confirm that the pgl3 basic vector and cmv promoter both contain sequences that are transactivated by glucocorticoid treatment. In our efforts to characterize the regulation of the ov gene by steroid hormones, we have repeatedly observed that many vector backbones and promoters are.
The backbone of the pgl2 luciferase reporter vectors was redesigned for the pgl3 vectors. To determine whether this sequence confers promoter activity, several cell lines including tc7 were transiently transfected with the pgl3bco1022 reporter data not shown. The originals have been uploaded to and processed by this website, using only the tools made available in the vectorization. Disruption of a novel regulatory element in the erythroid. Myogenic basic helixloophelix proteins regulate the. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. The pgl3 promoter vector contains the sv40 promoter but not the enhancer. This vector is useful in the study of functional promoter elements to regulate gene. To convert pdf to vector format, it is necessary to convert a pdf to bitmap image firstly and then you can easily convert the images to vectors. International journal of scientific and research publications ijsrp. We used the dualluciferase reporter assay system promega to measure the activity of the firefly luciferase gene and the renilla luciferase gene.
To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. To confirm the transcriptional origin of the regulation of mir155 under tnf. Pdf comparison of the responsiveness of the pgl3 and pgl4. Obesityassociated inflammation induces microrna155. Briefly, hela cells expressing empty pgl3 vector or other reporter constructs 600 ng and empty. The human gene for liver and red blood cellspecific pk pklr is located on chromosome 1q21 3 where it directs tissuespecific transcription for both the liverspecific isozymes pkl and the red blood cellspecific isozyme pkr46 by the use of alternate promoters. A vector file is a file illustrator, corel draw that can be opened and. The system comprises complex carbohydrate structures that are biosynthesized by the a and b transferases encoded by the a and b genes, respectively.
Luciferase activity in cells transfected with gfp and pgc1. Risk snpmediated promoterenhancer switching drives. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Promega pgl3 luciferase reporter vectors pgl3 basic vector.
Expression of abo bloodgroup genes is dependent upon an. The backbone of the pgl2 luciferase reporter vectors was redesigned for the pgl3 vectors for increased expression, with a modified coding region for. This is a free resource for the scientific community that is compiled by addgene. Isolation and cloning of human nqo1 promoter in pgl3 basic vector asma chinigarzadeh, razauden zulkifli, iman yaze and reyhaneh rahnamai tajadod. Luciferase signals were normalized to renilla signals n 3. Characterization of 3untranslated region of the mouse. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. The system comprises complex carbohydrate structures that are biosynthesized by. Four firefly luciferase reporter vectors with a modified reporter gene and vector backbone. Pdf free vector art 144,375 free downloads vecteezy.
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